Human metapneumovirus detection in patients with severe acute respiratory syndrome.
Identifieur interne : 005F78 ( Main/Exploration ); précédent : 005F77; suivant : 005F79Human metapneumovirus detection in patients with severe acute respiratory syndrome.
Auteurs : Paul K S. Chan [République populaire de Chine] ; John S. Tam ; Ching-Wan Lam ; Edward Chan ; Alan Wu ; Chi-Kong Li ; Thomas A. Buckley ; King-Cheung Ng ; Gavin M. Joynt ; Frankie W T. Cheng ; Ka-Fai To ; Nelson Lee ; David S C. Hui ; Jo L K. Cheung ; Ida Chu ; Esther Liu ; Sydney S C. Chung ; Joseph J Y. SungSource :
- Emerging infectious diseases [ 1080-6040 ] ; 2003.
Descripteurs français
- KwdFr :
- Adulte, Adulte d'âge moyen, Animaux, Anticorps antiviraux (isolement et purification), Cellules cultivées, Coronavirus (isolement et purification), Femelle, Flambées de maladies, Hong Kong (épidémiologie), Humains, Macaca mulatta, Maladies transmissibles émergentes (physiopathologie), Maladies transmissibles émergentes (virologie), Maladies transmissibles émergentes (épidémiologie), Metapneumovirus (immunologie), Metapneumovirus (isolement et purification), Mâle, RT-PCR, Sujet âgé, Syndrome respiratoire aigu sévère (physiopathologie), Syndrome respiratoire aigu sévère (virologie), Syndrome respiratoire aigu sévère (épidémiologie).
- MESH :
- immunologie : Metapneumovirus.
- isolement et purification : Anticorps antiviraux, Coronavirus, Metapneumovirus.
- physiopathologie : Maladies transmissibles émergentes, Syndrome respiratoire aigu sévère.
- virologie : Maladies transmissibles émergentes, Syndrome respiratoire aigu sévère.
- épidémiologie : Hong Kong, Maladies transmissibles émergentes, Syndrome respiratoire aigu sévère.
- Adulte, Adulte d'âge moyen, Animaux, Cellules cultivées, Femelle, Flambées de maladies, Humains, Macaca mulatta, Mâle, RT-PCR, Sujet âgé.
- Wicri :
- geographic : Hong Kong.
English descriptors
- KwdEn :
- Adult, Aged, Animals, Antibodies, Viral (isolation & purification), Cells, Cultured, Communicable Diseases, Emerging (epidemiology), Communicable Diseases, Emerging (physiopathology), Communicable Diseases, Emerging (virology), Coronavirus (isolation & purification), Disease Outbreaks, Female, Hong Kong (epidemiology), Humans, Macaca mulatta, Male, Metapneumovirus (immunology), Metapneumovirus (isolation & purification), Middle Aged, Reverse Transcriptase Polymerase Chain Reaction, Severe Acute Respiratory Syndrome (epidemiology), Severe Acute Respiratory Syndrome (physiopathology), Severe Acute Respiratory Syndrome (virology).
- MESH :
- chemical , isolation & purification : Antibodies, Viral.
- geographic , epidemiology : Hong Kong.
- epidemiology : Communicable Diseases, Emerging, Severe Acute Respiratory Syndrome.
- immunology : Metapneumovirus.
- isolation & purification : Coronavirus, Metapneumovirus.
- physiopathology : Communicable Diseases, Emerging, Severe Acute Respiratory Syndrome.
- virology : Communicable Diseases, Emerging, Severe Acute Respiratory Syndrome.
- Adult, Aged, Animals, Cells, Cultured, Disease Outbreaks, Female, Humans, Macaca mulatta, Male, Middle Aged, Reverse Transcriptase Polymerase Chain Reaction.
Abstract
We used a combination approach of conventional virus isolation and molecular techniques to detect human metapneumovirus (HMPV) in patients with severe acute respiratory syndrome (SARS). Of the 48 study patients, 25 (52.1%) were infected with HMPV; 6 of these 25 patients were also infected with coronavirus, and another 5 patients (10.4%) were infected with coronavirus alone. Using this combination approach, we found that human laryngeal carcinoma (HEp-2) cells were superior to rhesus monkey kidney (LLC-MK2) cells commonly used in previous studies for isolation of HMPV. These widely available HEp-2 cells should be included in conjunction with a molecular method for cell culture followup to detect HMPV, particularly in patients with SARS.
DOI: 10.3201/eid0909.030304
PubMed: 14519240
Affiliations:
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Le document en format XML
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Chan</name><affiliation wicri:level="1"><nlm:affiliation>Faculty of Medicine of the Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China. paulkschan@cuhk.edu.hk</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Faculty of Medicine of the Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR</wicri:regionArea><wicri:noRegion>Hong Kong SAR</wicri:noRegion></affiliation></author><author><name sortKey="Tam, John S" sort="Tam, John S" uniqKey="Tam J" first="John S" last="Tam">John S. Tam</name></author><author><name sortKey="Lam, Ching Wan" sort="Lam, Ching Wan" uniqKey="Lam C" first="Ching-Wan" last="Lam">Ching-Wan Lam</name></author><author><name sortKey="Chan, Edward" sort="Chan, Edward" uniqKey="Chan E" first="Edward" last="Chan">Edward Chan</name></author><author><name sortKey="Wu, Alan" sort="Wu, Alan" uniqKey="Wu A" first="Alan" last="Wu">Alan Wu</name></author><author><name sortKey="Li, Chi Kong" sort="Li, Chi Kong" uniqKey="Li C" first="Chi-Kong" last="Li">Chi-Kong Li</name></author><author><name sortKey="Buckley, Thomas A" sort="Buckley, Thomas A" uniqKey="Buckley T" first="Thomas A" last="Buckley">Thomas A. Buckley</name></author><author><name sortKey="Ng, King Cheung" sort="Ng, King Cheung" uniqKey="Ng K" first="King-Cheung" last="Ng">King-Cheung Ng</name></author><author><name sortKey="Joynt, Gavin M" sort="Joynt, Gavin M" uniqKey="Joynt G" first="Gavin M" last="Joynt">Gavin M. Joynt</name></author><author><name sortKey="Cheng, Frankie W T" sort="Cheng, Frankie W T" uniqKey="Cheng F" first="Frankie W T" last="Cheng">Frankie W T. Cheng</name></author><author><name sortKey="To, Ka Fai" sort="To, Ka Fai" uniqKey="To K" first="Ka-Fai" last="To">Ka-Fai To</name></author><author><name sortKey="Lee, Nelson" sort="Lee, Nelson" uniqKey="Lee N" first="Nelson" last="Lee">Nelson Lee</name></author><author><name sortKey="Hui, David S C" sort="Hui, David S C" uniqKey="Hui D" first="David S C" last="Hui">David S C. Hui</name></author><author><name sortKey="Cheung, Jo L K" sort="Cheung, Jo L K" uniqKey="Cheung J" first="Jo L K" last="Cheung">Jo L K. Cheung</name></author><author><name sortKey="Chu, Ida" sort="Chu, Ida" uniqKey="Chu I" first="Ida" last="Chu">Ida Chu</name></author><author><name sortKey="Liu, Esther" sort="Liu, Esther" uniqKey="Liu E" first="Esther" last="Liu">Esther Liu</name></author><author><name sortKey="Chung, Sydney S C" sort="Chung, Sydney S C" uniqKey="Chung S" first="Sydney S C" last="Chung">Sydney S C. Chung</name></author><author><name sortKey="Sung, Joseph J Y" sort="Sung, Joseph J Y" uniqKey="Sung J" first="Joseph J Y" last="Sung">Joseph J Y. Sung</name></author></analytic><series><title level="j">Emerging infectious diseases</title><idno type="ISSN">1080-6040</idno><imprint><date when="2003" type="published">2003</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult</term><term>Aged</term><term>Animals</term><term>Antibodies, Viral (isolation & purification)</term><term>Cells, Cultured</term><term>Communicable Diseases, Emerging (epidemiology)</term><term>Communicable Diseases, Emerging (physiopathology)</term><term>Communicable Diseases, Emerging (virology)</term><term>Coronavirus (isolation & purification)</term><term>Disease Outbreaks</term><term>Female</term><term>Hong Kong (epidemiology)</term><term>Humans</term><term>Macaca mulatta</term><term>Male</term><term>Metapneumovirus (immunology)</term><term>Metapneumovirus (isolation & purification)</term><term>Middle Aged</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Severe Acute Respiratory Syndrome (epidemiology)</term><term>Severe Acute Respiratory Syndrome (physiopathology)</term><term>Severe Acute Respiratory Syndrome (virology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adulte</term><term>Adulte d'âge moyen</term><term>Animaux</term><term>Anticorps antiviraux (isolement et purification)</term><term>Cellules cultivées</term><term>Coronavirus (isolement et purification)</term><term>Femelle</term><term>Flambées de maladies</term><term>Hong Kong (épidémiologie)</term><term>Humains</term><term>Macaca mulatta</term><term>Maladies transmissibles émergentes (physiopathologie)</term><term>Maladies transmissibles émergentes (virologie)</term><term>Maladies transmissibles émergentes (épidémiologie)</term><term>Metapneumovirus (immunologie)</term><term>Metapneumovirus (isolement et purification)</term><term>Mâle</term><term>RT-PCR</term><term>Sujet âgé</term><term>Syndrome respiratoire aigu sévère (physiopathologie)</term><term>Syndrome respiratoire aigu sévère (virologie)</term><term>Syndrome respiratoire aigu sévère (épidémiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" type="geographic" qualifier="epidemiology" xml:lang="en"><term>Hong Kong</term></keywords><keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Communicable Diseases, Emerging</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Metapneumovirus</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Metapneumovirus</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Coronavirus</term><term>Metapneumovirus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Anticorps antiviraux</term><term>Coronavirus</term><term>Metapneumovirus</term></keywords><keywords scheme="MESH" qualifier="physiopathologie" xml:lang="fr"><term>Maladies transmissibles émergentes</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="physiopathology" xml:lang="en"><term>Communicable Diseases, Emerging</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Maladies transmissibles émergentes</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Communicable Diseases, Emerging</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>Hong Kong</term><term>Maladies transmissibles émergentes</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adult</term><term>Aged</term><term>Animals</term><term>Cells, Cultured</term><term>Disease Outbreaks</term><term>Female</term><term>Humans</term><term>Macaca mulatta</term><term>Male</term><term>Middle Aged</term><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adulte</term><term>Adulte d'âge moyen</term><term>Animaux</term><term>Cellules cultivées</term><term>Femelle</term><term>Flambées de maladies</term><term>Humains</term><term>Macaca mulatta</term><term>Mâle</term><term>RT-PCR</term><term>Sujet âgé</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Hong Kong</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We used a combination approach of conventional virus isolation and molecular techniques to detect human metapneumovirus (HMPV) in patients with severe acute respiratory syndrome (SARS). Of the 48 study patients, 25 (52.1%) were infected with HMPV; 6 of these 25 patients were also infected with coronavirus, and another 5 patients (10.4%) were infected with coronavirus alone. Using this combination approach, we found that human laryngeal carcinoma (HEp-2) cells were superior to rhesus monkey kidney (LLC-MK2) cells commonly used in previous studies for isolation of HMPV. These widely available HEp-2 cells should be included in conjunction with a molecular method for cell culture followup to detect HMPV, particularly in patients with SARS.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Buckley, Thomas A" sort="Buckley, Thomas A" uniqKey="Buckley T" first="Thomas A" last="Buckley">Thomas A. Buckley</name><name sortKey="Chan, Edward" sort="Chan, Edward" uniqKey="Chan E" first="Edward" last="Chan">Edward Chan</name><name sortKey="Cheng, Frankie W T" sort="Cheng, Frankie W T" uniqKey="Cheng F" first="Frankie W T" last="Cheng">Frankie W T. Cheng</name><name sortKey="Cheung, Jo L K" sort="Cheung, Jo L K" uniqKey="Cheung J" first="Jo L K" last="Cheung">Jo L K. Cheung</name><name sortKey="Chu, Ida" sort="Chu, Ida" uniqKey="Chu I" first="Ida" last="Chu">Ida Chu</name><name sortKey="Chung, Sydney S C" sort="Chung, Sydney S C" uniqKey="Chung S" first="Sydney S C" last="Chung">Sydney S C. Chung</name><name sortKey="Hui, David S C" sort="Hui, David S C" uniqKey="Hui D" first="David S C" last="Hui">David S C. Hui</name><name sortKey="Joynt, Gavin M" sort="Joynt, Gavin M" uniqKey="Joynt G" first="Gavin M" last="Joynt">Gavin M. Joynt</name><name sortKey="Lam, Ching Wan" sort="Lam, Ching Wan" uniqKey="Lam C" first="Ching-Wan" last="Lam">Ching-Wan Lam</name><name sortKey="Lee, Nelson" sort="Lee, Nelson" uniqKey="Lee N" first="Nelson" last="Lee">Nelson Lee</name><name sortKey="Li, Chi Kong" sort="Li, Chi Kong" uniqKey="Li C" first="Chi-Kong" last="Li">Chi-Kong Li</name><name sortKey="Liu, Esther" sort="Liu, Esther" uniqKey="Liu E" first="Esther" last="Liu">Esther Liu</name><name sortKey="Ng, King Cheung" sort="Ng, King Cheung" uniqKey="Ng K" first="King-Cheung" last="Ng">King-Cheung Ng</name><name sortKey="Sung, Joseph J Y" sort="Sung, Joseph J Y" uniqKey="Sung J" first="Joseph J Y" last="Sung">Joseph J Y. Sung</name><name sortKey="Tam, John S" sort="Tam, John S" uniqKey="Tam J" first="John S" last="Tam">John S. Tam</name><name sortKey="To, Ka Fai" sort="To, Ka Fai" uniqKey="To K" first="Ka-Fai" last="To">Ka-Fai To</name><name sortKey="Wu, Alan" sort="Wu, Alan" uniqKey="Wu A" first="Alan" last="Wu">Alan Wu</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Chan, Paul K S" sort="Chan, Paul K S" uniqKey="Chan P" first="Paul K S" last="Chan">Paul K S. Chan</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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